P 178 Optimal storage temperature for human corneas in organ-culture

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P 178 Optimal storage temperature for human corneas in organ-culture

Purpose : The aim of this study is to compare the results of two series of penetrating keratoplasties, e.sch with a different method of conservation. Methods : The corneas of sixteen donors were conserved one of each pair at 4OC and the other at 31%. The average duration of conservation was 3.2 days at 4°C and 13.4 days at 31°C. Average endothelial cell density evaluated with trypan blue was 34...

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4211 Influence of donor and storage factors on quality of corneas stored by organ culture

Logistic regression methods were used to analyse the influence of donor and storage factors on the quality of c~meas stored by organ culture in the Bristol eve bank in 1991. Averaae donor a(18 was 57 vears fsd 22. n=2770) -and time from death to en~cleation v& 8 hours (&I 7, n&782): Corneas were placed into organ culture within 27 houra (sd 10, n+2550) of donor death and were stored for 22 days...

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Evaluation of potential organ culture media for eye banking using human donor corneas.

AIM To evaluate the ability of different commercially available cell culture solutions to preserve human donor corneas during 3 weeks of "closed system" organ culture at physiological temperature. This screening was performed in an attempt to establish a rational basis for the development of a serum-free organ culture medium for eye banking. METHODS 72 normal human donor corneas were organ cu...

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P 177 Does the organ-culture of human corneas lead to uniform capabilities of the stromal keratocytes?

Purpose : The aim of this study is to compare the results of two series of penetrating keratoplasties, e.sch with a different method of conservation. Methods : The corneas of sixteen donors were conserved one of each pair at 4OC and the other at 31%. The average duration of conservation was 3.2 days at 4°C and 13.4 days at 31°C. Average endothelial cell density evaluated with trypan blue was 34...

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Screening human donor corneas during organ culture for the presence of guttae.

AIMS To detect the presence of guttae by means of light microscopy during organ culture and to evaluate the influence of the presence of guttae in the donor tissue on transplantation outcome. METHODS Donor corneas were investigated for the presence of guttae by means of light microscopy at the end of organ culture. Recipient corneal buttons from patients with severe Fuchs' dystrophy and donor...

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ژورنال

عنوان ژورنال: Vision Research

سال: 1995

ISSN: 0042-6989

DOI: 10.1016/0042-6989(95)90494-8